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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RHOG-Specific Polyclonal antibody

RHOG-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19671-1-AP

Synonyms

RHOG, ARHG, Rho-related GTP-binding protein RhoG

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of MDA-MB-453s using 19671-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of C6 using 19671-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of Neuro-2a using 19671-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of NIH/3T3 using 19671-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of MCF-7 using 19671-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RHOG-Specific Polyclonal antibody (19671-1-AP)

    WB analysis of HeLa using 19671-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RHOG-Specific Polyclonal antibody (19671-1-AP)

    IHC staining of mouse kidney using 19671-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RHOG-Specific Polyclonal antibody (19671-1-AP)

    IHC staining of mouse kidney using 19671-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, C6 cells, HeLa cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19671-1-AP targets RHOG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member G (rho G)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberNM_001665
Gene symbol RHOG
Gene ID (NCBI) 391
RRIDAB_2878593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.

Protocols

Product Specific Protocols
WB protocol for RHOG-Specific antibody 19671-1-APDownload protocol
IHC protocol for RHOG-Specific antibody 19671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols