Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, pig brain tissue, fetal human brain tissue, Jurkat cells, A549 cells |
| Positive IHC detected in | mouse liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67213-1-Ig targets RHOQ/TC10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RHOQ/TC10 fusion protein Ag12078 相同性解析による交差性が予測される生物種 |
| Full Name | ras homolog gene family, member Q |
| Calculated molecular weight | 205 aa, 23 kDa |
| Observed molecular weight | 20-23 kDa |
| GenBank accession number | BC070485 |
| Gene Symbol | RHOQ/TC10 |
| Gene ID (NCBI) | 23433 |
| RRID | AB_2882505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17081 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHOQ/TC10 antibody 67213-1-Ig | Download protocol |
| IHC protocol for RHOQ/TC10 antibody 67213-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |