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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIRO1 Recombinant antibody (84055-5-RR)

    WB analysis using 84055-5-RR (same clone as 84055-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MIRO1 Recombinant antibody (84055-5-RR)

    IHC staining of mouse kidney using 84055-5-RR (same clone as 84055-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84055-5-RR

    Affinity and Kinetic Characterization of 84055-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

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MIRO1 Recombinant antibody, PBS Only

MIRO1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84055-5-PBS
Clone No.241157D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84055-5-PBS targets MIRO1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIRO1 fusion protein Ag15605 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member T1
Calculated molecular weight 618 aa, 71 kDa
Observed molecular weight75 kDa
GenBank accession numberBC060781
Gene Symbol MIRO1
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXI2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.