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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIRO1 Recombinant antibody, PBS Only

MIRO1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241157D1

Cat no : 84055-5-PBS

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIRO1 Recombinant antibody (84055-5-RR)

    WB analysis using 84055-5-RR (same clone as 84055-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MIRO1 Recombinant antibody (84055-5-RR)

    IHC staining of mouse kidney using 84055-5-RR (same clone as 84055-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84055-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84055-5-RR

    Affinity and Kinetic Characterization of 84055-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84055-5-PBS is MIRO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIRO1 fusion protein Ag15605 相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member T1
Calculated molecular weight 618 aa, 71 kDa
Observed molecular weight75 kDa
GenBank accession numberBC060781
Gene symbol MIRO1
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.