Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HepG2 cells |
| Positive IHC detected in | human colon tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RHPN2 fusion protein Ag3353 相同性解析による交差性が予測される生物種 |
| Full Name | rhophilin, Rho GTPase binding protein 2 |
| Calculated molecular weight | 686 aa, 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC036447 |
| Gene Symbol | RHPN2 |
| Gene ID (NCBI) | 85415 |
| RRID | AB_2877872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RHPN2 binds specifically to GTP-Rho. It may function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity. The MW of this protein is 77 kDa, and this antibody specially recognises the 77 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| IHC protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| IF protocol for RHPN2 antibody 12671-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. | ||
Cell Cycle P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.
| ||
J Biochem Mol Toxicol Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179 |