RIC8A Polyclonal antibody
RIC8A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18707-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
18707-1-AP targets RIC8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC011821 |
| Gene symbol | RIC8A |
| Gene ID (NCBI) | 60626 |
| RRID | AB_2179831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIC8A antibody 18707-1-AP | Download protocol |
| IHC protocol for RIC8A antibody 18707-1-AP | Download protocol |
| IF protocol for RIC8A antibody 18707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Dyn Nucleotide exchange factor RIC-8 is indispensable in mammalian early development. |
