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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIC8A Monoclonal antibody, PBS Only

RIC8A Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66625-1-PBS

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RIC8A Monoclonal antibody (66625-1-Ig)

    WB analysis of HeLa using 66625-1-Ig (same clone as 66625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66625-1-Ig (RIC8A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RIC8A Monoclonal antibody (66625-1-Ig)

    IP experiment of HEK-293 using 66625-1-Ig (same clone as 66625-1-PBS)

    IP result of anti-RIC8A (IP:66625-1-Ig, 5ug; Detection:66625-1-Ig 1:2000) with HEK-293 cells lysate 4000 ug. This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IHC staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IHC staining of human pancreas using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IHC staining of human pancreas using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66625-1-Ig (RIC8A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using RIC8A Monoclonal antibody (66625-1-Ig)

    IF Staining of mouse brain using 66625-1-Ig (same clone as 66625-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RIC8A antibody (66625-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66625-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66625-1-PBS targets RIC8A in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RIC8A fusion protein Ag1593 相同性解析による交差性が予測される生物種
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated molecular weight 60 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011821
Gene symbol RIC8A
Gene ID (NCBI) 60626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.