Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 27248-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 27248-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 27248-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 27248-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 27248-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 27248-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 27248-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 27248-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RICTOR antibody (27248-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of NIH/3T3 using 27248-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (27248-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 27248-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27248-1-AP (RICTOR antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

RICTOR Polyclonal antibody

RICTOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27248-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells
Positive IHC detected in	human lung cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 21 publications below
IHC	See 1 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

27248-1-AP targets RICTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RICTOR fusion protein Ag25649 相同性解析による交差性が予測される生物種
Full Name	rapamycin-insensitive companion of mTOR
Calculated molecular weight	192 kDa
Observed molecular weight	192 kDa
GenBank accession number	BC029608
Gene Symbol	RICTOR
Gene ID (NCBI)	253260
RRID	AB_2880817
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6R327
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RICTOR antibody 27248-1-AP	Download protocol
IHC protocol for RICTOR antibody 27248-1-AP	Download protocol
IF protocol for RICTOR antibody 27248-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB	Aging (Albany NY) Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts Authors - Shi-Yu Wang View Article
mouse	WB,IF	Cell Rep Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways Authors - Tatsuki Yasuda View Article
mouse	IF	J Cell Physiol Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes Authors - Rui Hua View Article
human	WB	J Ethnopharmacol Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway Authors - Jie-Ru Yu View Article
human	WB	Cell Commun Signal Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy. Authors - Norihiko Sasaki View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27248-1-AP

WB analysis using 27248-1-AP

WB analysis of NIH/3T3 using 27248-1-AP

IHC staining of human lung cancer using 27248-1-AP

IHC staining of human lung cancer using 27248-1-AP

IHC staining of mouse testis using 27248-1-AP

IHC staining of mouse testis using 27248-1-AP

IF Staining of HeLa using 27248-1-AP

IF Staining of NIH/3T3 using 27248-1-AP

IF Staining of HeLa using 27248-1-AP

RICTOR Polyclonal antibody

RICTOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A glycolytic shift in Schwann cells supports injured axons.

Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes

Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway

Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.