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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RICTOR Monoclonal antibody

RICTOR Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1C4

Cat no : 66867-2-Ig

Synonyms

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog, 1D1C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis using 66867-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66867-2-Ig (RICTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HEK-293 using 66867-2-Ig

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Western Blot (WB) analysis of HEK-293 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HEK-293 using 66867-2-Ig

    Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Western Blot (WB) analysis of HeLa cells using RICTOR Monoclonal antibody (66867-2-Ig)

    WB analysis of HeLa using 66867-2-Ig

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RICTOR Monoclonal antibody (66867-2-Ig)

    IF Staining of NIH/3T3 using 66867-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (66867-2-Ig, Clone: 1D1C4 ) at dilution of 1:1700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inNCI-H1299 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:850-1:3400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

66867-2-Ig targets RICTOR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RICTOR fusion protein Ag28487 相同性解析による交差性が予測される生物種
Full Name rapamycin-insensitive companion of mTOR
Calculated molecular weight 192 kDa
Observed molecular weight 192 kDa
GenBank accession numberBC029608
Gene symbol RICTOR
Gene ID (NCBI) 253260
RRIDAB_2882204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RICTOR, is a key component of the mTOR complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. RICTOR is the upstream kinase of several AGC kinase family members including AKT, SGK, S6K mutants and several PKC isoforms. Activation of RICTOR-mTORC2 modifies actin organization and promotes cell proliferation and survival. Rictor is overexpressed in several cancers leading to hyperactive mTORC2 and has been shown to play a causal role in glioma formation. Rictor expression has been demonstrated to be regulated transcriptionally and via protein degradation.

Protocols

Product Specific Protocols
WB protocol for RICTOR antibody 66867-2-IgDownload protocol
IF protocol for RICTOR antibody 66867-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells

Authors - Lixin He
View Article
humanWB

Front Mol Biosci

FLCN Regulates HIF2α Nuclear Import and Proliferation of Clear Cell Renal Cell Carcinoma.

Authors - Xuyang Zhao
View Article
humanWB

Int J Mol Sci

KLF4 Suppresses the Progression of Hepatocellular Carcinoma by Reducing Tumor ATP Synthesis through Targeting the Mir-206/RICTOR Axis

Authors - Yongjin Wang
View Article
humanWB

Oncogene

UBXN2A suppresses the Rictor-mTORC2 signaling pathway, an established tumorigenic pathway in human colorectal cancer

Authors - Sanam Sane
View Article