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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIF1 Polyclonal antibody

RIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30119-1-AP

Synonyms

DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIF1 Polyclonal antibody (30119-1-AP)

    WB analysis using 30119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30119-1-AP (RIF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of human cervical cancer using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of human cervical cancer using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of mouse testis using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIF1 Polyclonal antibody (30119-1-AP)

    IF Staining of HeLa using 30119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RIF1 antibody (30119-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RIF1 Polyclonal antibody (30119-1-AP)

    IF Staining of HEK-293 using 30119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RIF1 antibody (30119-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, HEK-293T cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30119-1-AP targets RIF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIF1 fusion protein Ag32422 相同性解析による交差性が予測される生物種
Full Name RAP1 interacting factor homolog (yeast)
Calculated molecular weight274 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001177663.1
Gene symbol RIF1
Gene ID (NCBI) 55183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

Protocols

Product Specific Protocols
WB protocol for RIF1 antibody 30119-1-APDownload protocol
IHC protocol for RIF1 antibody 30119-1-APDownload protocol
IF protocol for RIF1 antibody 30119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols