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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    WB analysis using 20566-1-AP (same clone as 20566-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20566-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    WB analysis of A431 using 20566-1-AP (same clone as 20566-1-PBS)

    WB result of RIG-1/DDX58 antibody (20566-1-AP; 1:2000; room temperature for 1.5 hours) with wild-type and RIG-1/DDX58 knockout A431 cells. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    WB analysis of MCF-7 using 20566-1-AP (same clone as 20566-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    WB analysis of mouse brain using 20566-1-AP (same clone as 20566-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    WB analysis of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    IP experiment of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

    IP result of anti-RIG-1/DDX58 (IP:20566-1-AP, 4ug; Detection:20566-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using RIG-1/DDX58 Polyclonal antibody (20566-1-AP)

    IF Staining of H9C2 using 20566-1-AP (same clone as 20566-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using RIG-1/DDX58 antibody (20566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

RIG-1/DDX58 Polyclonal antibody, PBS Only

RIG-1/DDX58 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20566-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

DDX58, RIG-I, Retinoic acid-inducible gene 1 protein, EC:3.6.4.13, DEAD box protein 58

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20566-1-PBS targets RIG-1/DDX58 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 106 kDa
Observed molecular weight 101~106 kDa
GenBank accession numberNM_014314
Gene Symbol RIG-1/DDX58
Gene ID (NCBI) 23586
RRIDAB_10700006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.