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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIG-1/DDX58 Monoclonal antibody, PBS Only

RIG-1/DDX58 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10F5

Cat no : 67556-1-PBS

Synonyms

DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIG-1/DDX58 Monoclonal antibody (67556-1-Ig)

    WB analysis using 67556-1-Ig (same clone as 67556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67556-1-Ig (DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RIG-1/DDX58 Monoclonal antibody (67556-1-Ig)

    IF Staining of HepG2 using 67556-1-Ig (same clone as 67556-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIG-1/DDX58 antibody (67556-1-Ig, Clone: 3B10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67556-1-PBS targets RIG-1/DDX58 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RIG-1/DDX58 fusion protein Ag18591 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 925 aa, 106 kDa
Observed molecular weight 101/106 kDa
GenBank accession numberBC132786
Gene symbol RIG-1/DDX58
Gene ID (NCBI) 23586
RRIDAB_2882770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.