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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIG-1/DDX58 Recombinant antibody

RIG-1/DDX58 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242424E7

Cat no : 84861-5-RR

Synonyms

DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using RIG-1/DDX58 Recombinant antibody (84861-5-RR)

    WB analysis of A431 using 84861-5-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RIG-1/DDX58 Recombinant antibody (84861-5-RR)

    WB analysis using 84861-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84861-5-RR

    Affinity and Kinetic Characterization of 84861-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84861-5-RR against Human RIG-1/DDX58 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA431 cells, THP-1 cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84861-5-RR targets RIG-1/DDX58 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RIG-1/DDX58 fusion protein Ag18585 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 925 aa, 106 kDa
Observed molecular weight 101~106 kDa
GenBank accession numberBC132786
Gene symbol RIG-1/DDX58
Gene ID (NCBI) 23586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

Protocols

Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 84861-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols