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  • KD/KO Validated

RILP Polyclonal antibody

RILP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA, PLA

Conjugate

Unconjugated

Cat no : 13574-1-AP

Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193


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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13574-1-AP targets RILP in WB, IHC, IF/ICC, ELISA, PLA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RILP fusion protein Ag4472 相同性解析による交差性が予測される生物種
Full Name Rab interacting lysosomal protein
Calculated molecular weight 401 aa, 41 kDa
Observed molecular weight 41-45 kDa
GenBank accession numberBC031621
Gene symbol RILP
Gene ID (NCBI) 83547
RRIDAB_2877959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RILP antibody 13574-1-APDownload protocol
IHC protocol for RILP antibody 13574-1-APDownload protocol
IF protocol for RILP antibody 13574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

Authors - Rahul Kumar
humanPLA

J Extracell Vesicles

Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles.

Authors - Xuefeng Fei
humanWB

J Exp Clin Cancer Res

Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.

Authors - Minghui Chang
humanWB

J Biol Chem

A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.

Authors - Mai E Oguchi
  • KD Validated
WB

Cell Death Dis

TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.

Authors - Jia Hu
humanWB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Q (Verified Customer) (03-22-2021)

There is weak band between 35 and 50 kD. Seems working with WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK lysate
RILP Antibody Western Blot validation (1:500 dilution) in HEK lysate (Cat no:13574-1-AP)