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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RILP Recombinant antibody, PBS Only (Capture)

RILP Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230208G2

Cat no : 82996-4-PBS

Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RILP Recombinant antibody (82996-4-RR)

    WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00025-1

    Cytometric bead array standard curve of MP00025-1

    Cytometric bead array standard curve of MP00025-1, RILP Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82996-4-PBS. Detection antibody: 82996-3-PBS. Standard: Ag4472. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82996-4-PBS targets RILP as part of a matched antibody pair:

MP00025-1: 82996-4-PBS capture and 82996-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
Immunogen RILP fusion protein Ag4472 相同性解析による交差性が予測される生物種
Full Name Rab interacting lysosomal protein
Calculated molecular weight 401 aa, 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC031621
Gene symbol RILP
Gene ID (NCBI) 83547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.