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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RING1 Polyclonal antibody

RING1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15037-1-AP

Synonyms

Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RING1 Polyclonal antibody (15037-1-AP)

    WB analysis of mouse skeletal muscle using 15037-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using RING1 Polyclonal antibody (15037-1-AP)

    WB analysis of BxPC-3 using 15037-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RING1 Polyclonal antibody (15037-1-AP)

    WB analysis of PC-3 using 15037-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RING1 Polyclonal antibody (15037-1-AP)

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RING1 Polyclonal antibody (15037-1-AP)

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, BxPC-3 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15037-1-AP targets RING1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RING1 fusion protein Ag6763 相同性解析による交差性が予測される生物種
Full Name ring finger protein 1
Calculated molecular weight 42 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002922
Gene symbol RING1
Gene ID (NCBI) 6015
RRIDAB_2177964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ring1, also named as Ring1a, constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It's an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

Protocols

Product Specific Protocols
WB protocol for RING1 antibody 15037-1-APDownload protocol
IHC protocol for RING1 antibody 15037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols