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- KD/KO Validated
RIOK1 Polyclonal antibody
RIOK1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 17222-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RIOK1 fusion protein Ag11057 相同性解析による交差性が予測される生物種 |
| Full Name | RIO kinase 1 (yeast) |
| Calculated molecular weight | 568 aa, 66 kDa |
| Observed molecular weight | 75-80 kDa |
| GenBank accession number | BC006104 |
| Gene symbol | RIOK1 |
| Gene ID (NCBI) | 83732 |
| RRID | AB_2284990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| IP protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. | ||
iScience Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion. | ||
Cancer Res Treat Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression. |
