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KD/KO Validated

RIOK3 Polyclonal antibody

Name: RIOK3 Polyclonal antibody
Brand: Proteintech
SKU: 13593-1-AP

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13593-1-AP

Synonyms

sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13593-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13593-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 13593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 13593-1-AP
IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug.
IHC staining of human breast cancer using 13593-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13593-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 13593-1-AP
Immunofluorescent analysis of MCF-7 cells using 13593-1-AP (RIOK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13593-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, mouse testis tissue, MCF-7 cells, rat testis tissue, HeLa cells, Jurkat cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

13593-1-AP targets RIOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RIOK3 fusion protein Ag4497 相同性解析による交差性が予測される生物種
Full Name	RIO kinase 3 (yeast)
Calculated molecular weight	516 aa, 60 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC039729
Gene symbol	RIOK3
Gene ID (NCBI)	8780
RRID	AB_2178105
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.

Protocols

Product Specific Protocols
WB protocol for RIOK3 antibody 13593-1-AP	Download protocol
IHC protocol for RIOK3 antibody 13593-1-AP	Download protocol
IF protocol for RIOK3 antibody 13593-1-AP	Download protocol
IP protocol for RIOK3 antibody 13593-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. Authors - Nandan S Gokhale View Article
mouse	WB	Genes Dev miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Authors - Lingbo Zhang View Article
human	WB	Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. Authors - Min Yu View Article
mouse	WB	Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation. Authors - Yong Shen KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIOK3 Polyclonal antibody

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13593-1-AP

WB analysis of mouse testis using 13593-1-AP

WB analysis of MCF-7 using 13593-1-AP

WB analysis using 13593-1-AP

IP experiment of mouse testis using 13593-1-AP

IHC staining of human breast cancer using 13593-1-AP

IHC staining of human breast cancer using 13593-1-AP

IF Staining of MCF-7 using 13593-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1.

RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis.

Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.