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  • KD/KO Validated

RIPK2 Polyclonal antibody

RIPK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15366-1-AP

Synonyms

CARD3, CARDIAK, CCK, GIG30, Receptor interacting protein 2, RICK, RIP 2, RIP2, RIPK2


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Tested Applications

Positive WB detected inHEK-293T cells, mouse brain tissue, HeLa cells, THP-1 cells
Positive IP detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15366-1-AP targets RIPK2 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIPK2 fusion protein Ag0909 相同性解析による交差性が予測される生物種
Full Name receptor-interacting serine-threonine kinase 2
Calculated molecular weight 61 kDa
Observed molecular weight 46-55 kDa, 61-72 kDa
GenBank accession numberBC004553
Gene symbol RIPK2
Gene ID (NCBI) 8767
RRIDAB_2253650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for RIPK2 antibody 15366-1-APDownload protocol
IP protocol for RIPK2 antibody 15366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Antioxidants (Basel)

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Authors - Menghui Xu
humanWB

Obesity (Silver Spring)

Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.

Authors - Yanping Wang
humanWB

J Pharmacol Exp Ther

Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.

Authors - Mohamed Salla
  • KD Validated
mouseWB

Front Cell Infect Microbiol

Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.

Authors - Chun-Xia Shi
mouseWB,IP

Immunol Lett

A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation.

Authors - Dong-Yi Cao
humanWB

Front Genet

Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.

Authors - Qian Yang
  • KD Validated