RIP3 Polyclonal antibody
RIP3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 29080-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29080-1-AP targets RIP3 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RIP3 fusion protein Ag30337 相同性解析による交差性が予測される生物種 |
Full Name | receptor-interacting serine-threonine kinase 3 |
Calculated molecular weight | 518 aa, 57 kDa |
Observed molecular weight | 57 kDa |
GenBank accession number | BC062584 |
Gene symbol | RIPK3 |
Gene ID (NCBI) | 11035 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIPK3,also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).
Protocols
Product Specific Protocols | |
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WB protocol for RIP3 antibody 29080-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |