Validation Data Gallery
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Tested Applications
| Positive IP detected in | SKOV-3 cells |
| Positive IHC detected in | human lung cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26458-1-AP targets RIPK4 in IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RIPK4 fusion protein Ag24202 相同性解析による交差性が予測される生物種 |
| Full Name | receptor-interacting serine-threonine kinase 4 |
| Calculated molecular weight | 784 aa, 86 kDa |
| GenBank accession number | BC110617 |
| Gene Symbol | RIPK4 |
| Gene ID (NCBI) | 54101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T11 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |