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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIT1 Polyclonal antibody (26977-1-AP)

    WB analysis using 26977-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26977-1-AP (RIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RIT1 Polyclonal antibody (26977-1-AP)

    IF Staining of MCF-7 using 26977-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIT1 antibody (26977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RIT1 Polyclonal antibody

RIT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GTP binding protein Rit1, Ras like protein expressed in many tissues, Ras like without CAAX 1, Ras like without CAAX protein 1, RIBB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, human placenta tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26977-1-AP targets RIT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIT1 fusion protein Ag25636 相同性解析による交差性が予測される生物種
Full Name Ras-like without CAAX 1
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC104186
Gene Symbol RIT1
Gene ID (NCBI) 6016
RRIDAB_3669578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92963
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).

Protocols

Product Specific Protocols
WB protocol for RIT1 antibody 26977-1-APDownload protocol
IF protocol for RIT1 antibody 26977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols