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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIT2 Polyclonal antibody

RIT2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12473-1-AP

Synonyms

GTP binding protein Rit2, Ras like without CAAX 2, RIBA, RIN, RIT2, ROC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIT2 Polyclonal antibody (12473-1-AP)

    WB analysis using 12473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RIT2 Polyclonal antibody (12473-1-AP)

    IP experiment of mouse brain using 12473-1-AP

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug.

  • Immunoprecipitation (IP) experiment of rat brain tissue using RIT2 Polyclonal antibody (12473-1-AP)

    IP experiment of rat brain using 12473-1-AP

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RIT2 Polyclonal antibody (12473-1-AP)

    IHC staining of mouse brain using 12473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RIT2 Polyclonal antibody (12473-1-AP)

    IHC staining of mouse pancreas using 12473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12473-1-AP targets RIT2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIT2 fusion protein Ag3160 相同性解析による交差性が予測される生物種
Full Name Ras-like without CAAX 2
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC018060
Gene symbol RIT2
Gene ID (NCBI) 6014
RRIDAB_3669140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).

Protocols

Product Specific Protocols
WB protocol for RIT2 antibody 12473-1-APDownload protocol
IHC protocol for RIT2 antibody 12473-1-APDownload protocol
IP protocol for RIT2 antibody 12473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols