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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RIT2 Recombinant antibody, PBS Only (Detector)

RIT2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241916A7

Cat no : 84520-1-PBS

Synonyms

Ras like without CAAX protein 2, Ras like protein expressed in neurons, GTP-binding protein Rit2, GTP binding protein Rit2, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIT2 Recombinant antibody (84520-1-RR)

    WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84520-1-RR (RIT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84520-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1

    Cytometric bead array standard curve of MP01369-1, RIT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84520-2-PBS. Detection antibody: 84520-1-PBS. Standard: Ag3160. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84520-1-RR

    Affinity and Kinetic Characterization of 84520-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84520-1-RR against Human RIT2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84520-1-PBS targets RIT2 as part of a matched antibody pair:

MP01369-1: 84520-2-PBS capture and 84520-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RIT2 fusion protein Ag3160 相同性解析による交差性が予測される生物種
Full Name Ras-like without CAAX 2
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC018060
Gene symbol RIT2
Gene ID (NCBI) 6014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).