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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RMND5B Polyclonal antibody

RMND5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25803-1-AP

Synonyms

Protein RMD5 homolog B, RMND5B

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RMND5B Polyclonal antibody (25803-1-AP)

    WB analysis of A549 using 25803-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25803-1-AP (RMND5B Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human colon tissue using RMND5B Polyclonal antibody (25803-1-AP)

    IHC staining of human colon using 25803-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using RMND5B Polyclonal antibody (25803-1-AP)

    IHC staining of human colon using 25803-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RMND5B Polyclonal antibody (25803-1-AP)

    IHC staining of human gliomas using 25803-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25803-1-AP (RMND5B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RMND5B Polyclonal antibody (25803-1-AP)

    IF Staining of A549 using 25803-1-AP

    Immunofluorescent analysis of A549 cells using 25803-1-AP (RMND5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25803-1-AP is RMND5B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RMND5B fusion protein Ag22714 相同性解析による交差性が予測される生物種
Full Name required for meiotic nuclear division 5 homolog B (S. cerevisiae)
Calculated molecular weight 393 aa, 44 kDa
Observed molecular weight 40-44 kDa
GenBank accession numberBC009911
Gene symbol RMND5B
Gene ID (NCBI) 64777
RRIDAB_2880243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RMND5B antibody 25803-1-APDownload protocol
IHC protocol for RMND5B antibody 25803-1-APDownload protocol
IF protocol for RMND5B antibody 25803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

S (Verified Customer) (11-23-2021)

This antibody is quite good. I used it for KO validation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
RMND5B Antibody Western Blot validation (1:1000 dilution) in (Cat no:25803-1-AP)