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KD/KO Validated

RMP Polyclonal antibody

Name: RMP Polyclonal antibody
Brand: Proteintech
SKU: 11277-1-AP

RMP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11277-1-AP

Synonyms

C19orf2, NNX3, PPP1R19, Protein NNX3, RMP, RPB5 mediating protein, URI

Validation Data Gallery

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AllWBIPIHC

WB analysis of A549 using 11277-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11277-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11277-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11277-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11277-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11277-1-AP
IP result of anti-RMP (IP:11277-1-AP, 4ug; Detection:11277-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human lymphoma using 11277-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11277-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11277-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 11277-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lymphoma tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 6 publications below
IHC	See 5 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

11277-1-AP targets RMP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RMP fusion protein Ag1789 相同性解析による交差性が予測される生物種
Full Name	chromosome 19 open reading frame 2
Calculated molecular weight	65 kDa
Observed molecular weight	70-80 kDa
GenBank accession number	BC026184
Gene symbol	RMP
Gene ID (NCBI)	8725
RRID	AB_10596929
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RMP antibody 11277-1-AP	Download protocol
IHC protocol for RMP antibody 11277-1-AP	Download protocol
IP protocol for RMP antibody 11277-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers Authors - Zhiwen Ding KD Validated View Article
human	WB	Oncogene RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription. Authors - J Zhang KD Validated View Article
human	WB, IHC	Cell Death Dis URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription. Authors - J-L Fan KD Validated View Article
human	IHC	Oncotarget RMP predicts survival and adjuvant TACE response in hepatocellular carcinoma. Authors - Jian Zhang View Article
human	WB	Am J Cancer Res Silence of URI in gastric cancer cells promotes cisplatin-induced DNA damage and apoptosis Authors - Huiqin Bian KD Validated View Article
human	WB,IHC	Cardiovasc Res Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure. Authors - Jian Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RMP Polyclonal antibody

RMP Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of A549 using 11277-1-AP

WB analysis of Jurkat using 11277-1-AP

WB analysis of HEK-293 using 11277-1-AP

WB analysis of HeLa using 11277-1-AP

WB analysis of HepG2 using 11277-1-AP

IP experiment of HeLa using 11277-1-AP

IHC staining of human lymphoma using 11277-1-AP

IHC staining of human liver cancer using 11277-1-AP

IHC staining of human liver cancer using 11277-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.

URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription.

RMP predicts survival and adjuvant TACE response in hepatocellular carcinoma.

Silence of URI in gastric cancer cells promotes cisplatin-induced DNA damage and apoptosis

Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure.