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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASE2/EDN Polyclonal antibody

RNASE2/EDN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18172-1-AP

Synonyms

RNASE2, RNase UpI-2, RNase 2, Non-secretory ribonuclease, Eosinophil-derived neurotoxin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASE2 Polyclonal antibody (18172-1-AP)

    WB analysis using 18172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18172-1-AP (RNASE2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RNASE2/EDN Polyclonal antibody (18172-1-AP)

    IHC staining of human colon using 18172-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18172-1-AP (RNASE2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman urine sample
Positive IHC detected inhuman normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18172-1-AP targets RNASE2/EDN in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASE2/EDN fusion protein Ag12834 相同性解析による交差性が予測される生物種
Full Name ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
Calculated molecular weight 161 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC093678
Gene symbol RNASE2
Gene ID (NCBI) 6036
RRIDAB_3669293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNASE2/EDN antibody 18172-1-APDownload protocol
IHC protocol for RNASE2/EDN antibody 18172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols