Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
55338-1-AP targets RNASE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ribonuclease, RNase A family, 3 (eosinophil cationic protein) |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | NM_002935 |
| Gene Symbol | RNASE3 |
| Gene ID (NCBI) | 6037 |
| RRID | AB_2881312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12724 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| IHC protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| IF protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Allergy Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity | ||
Cell Commun Signal Allergen-induced CD11c + dendritic cell pyroptosis aggravates allergic rhinitis | ||
J Pharm Biomed Anal Network pharmacology analysis and experimental validation of Xiao-Qing-Long-Tang's therapeutic effects against neutrophilic asthma | ||
Int Immunopharmacol Androgens have therapeutic potential in T2 asthma by mediating METTL3 in bronchial epithelial cells |