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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASE4 Polyclonal antibody (30732-1-AP)

    WB analysis using 30732-1-AP (same clone as 30732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RNASE4 Polyclonal antibody (30732-1-AP)

    WB analysis using 30732-1-AP (same clone as 30732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using RNASE4 Polyclonal antibody (30732-1-AP)

    IHC staining of rat lung using 30732-1-AP (same clone as 30732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using RNASE4 Polyclonal antibody (30732-1-AP)

    IHC staining of rat lung using 30732-1-AP (same clone as 30732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using RNASE4 Polyclonal antibody (30732-1-AP)

    IHC staining of mouse bladder using 30732-1-AP (same clone as 30732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30732-1-PBS in a different storage buffer formulation.

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RNASE4 Polyclonal antibody, PBS Only

RNASE4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30732-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30732-1-PBS targets RNASE4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASE4 fusion protein Ag29272 相同性解析による交差性が予測される生物種
Full Name ribonuclease, RNase A family, 4
Observed molecular weight17-20 kDa
GenBank accession numberBC015520
Gene Symbol RNASE4
Gene ID (NCBI) 6038
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34096
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human Ribonuclease 4 (RNase 4), one of the eight members of the human RNase A superfamily of endoribonucleases, is a uridine-specific endoribonuclease with a preference to cleave RNA after uridine residues prior to purines (PMID: 33818125). RNase 4 is a newly identified host defense peptide in the human kidney and bladder and kills uropathogenic E. coli (UPEC) and multi-drug resistant UPEC (PMID: 28955965).