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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASE6 Polyclonal antibody, PBS Only

RNASE6 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16425-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using RNASE6 Polyclonal antibody (16425-1-AP)

    IHC staining of human liver using 16425-1-AP (same clone as 16425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RNASE6 Polyclonal antibody (16425-1-AP)

    IHC staining of human liver using 16425-1-AP (same clone as 16425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RNASE6 Polyclonal antibody (16425-1-AP)

    IHC staining of mouse heart using 16425-1-AP (same clone as 16425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RNASE6 Polyclonal antibody (16425-1-AP)

    IHC staining of mouse heart using 16425-1-AP (same clone as 16425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using RNASE6 Polyclonal antibody (16425-1-AP)

    IF Staining of THP-1 using 16425-1-AP (same clone as 16425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RNASE6 antibody (16425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16425-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16425-1-PBS targets RNASE6 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASE6 fusion protein Ag9667 相同性解析による交差性が予測される生物種
Full Name ribonuclease, RNase A family, k6
Calculated molecular weight 150 aa, 17 kDa
GenBank accession numberBC020848
Gene symbol RNASE6
Gene ID (NCBI) 6039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rnase6, a protein belonging to the superfamily, is considered the only vertebrate specific enzyme known to have a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction, and host defense. Rnase6 expression was upregulated in the peripheral blood and plaque tissues of AS patients (PMID: 34395402).