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  • KD/KO Validated

RNASEH1 Polyclonal antibody

RNASEH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15606-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, U2OS cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15606-1-AP targets RNASEH1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH1 fusion protein Ag7990 相同性解析による交差性が予測される生物種
Full Name ribonuclease H1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
RRIDAB_2238624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocols

Product Specific Protocols
WB protocol for RNASEH1 antibody 15606-1-APDownload protocol
IF protocol for RNASEH1 antibody 15606-1-APDownload protocol
IP protocol for RNASEH1 antibody 15606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.

Authors - Aparna Gorthi
humanWB

Cell

Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.

Authors - Luciano E Marasco
humanWB

Nature

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.

Authors - Vaibhav Bhatia
  • KD Validated
humanWB

Cell Res

Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.

Authors - Junyu Wu
mouseIF

Nat Cell Biol

Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.

Authors - Flavia Michelini
humanWB

Nat Commun

RNA-mediated double-strand break repair by end-joining mechanisms

Authors - Youngkyu Jeon
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