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  • Featured Product
  • KD/KO Validated

RNASEH1 Recombinant antibody

RNASEH1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C12

Cat no : 82771-1-RR

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH1 Recombinant antibody (82771-1-RR)

    WB analysis using 82771-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RNASEH1 Recombinant antibody (82771-1-RR)

    WB analysis of HeLa using 82771-1-RR

    WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RNASEH1 Recombinant antibody (82771-1-RR)

    IHC staining of human ovarian cancer using 82771-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82771-1-RR

    Affinity and Kinetic Characterization of 82771-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, U2OS cells, Jurkat cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82771-1-RR targets RNASEH1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNASEH1 fusion protein Ag7990 相同性解析による交差性が予測される生物種
Full Name ribonuclease H1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002973
Gene symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocols

Product Specific Protocols
WB protocol for RNASEH1 antibody 82771-1-RRDownload protocol
IHC protocol for RNASEH1 antibody 82771-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols