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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2A Monoclonal antibody (67976-1-Ig)

    WB analysis using 67976-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67976-1-Ig (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RNASEH2A Monoclonal antibody (67976-1-Ig)

    IHC staining of human skin cancer using 67976-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67976-1-Ig (RNASEH2A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RNASEH2A Monoclonal antibody

RNASEH2A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67976-1-Ig
Clone No.1A2E8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ribonuclease H2, subunit A, Ribonuclease H2 subunit A, JUNB, EC:3.1.26.4, Aicardi-Goutieres syndrome 4 protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Hela cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67976-1-Ig targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RNASEH2A fusion protein Ag9256 相同性解析による交差性が予測される生物種
Full Name ribonuclease H2, subunit A
Calculated molecular weight 299 aa, 33 kDa
GenBank accession numberBC011748
Gene Symbol RNASEH2A
Gene ID (NCBI) 10535
RRIDAB_2918725
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75792
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Protocols

Product Specific Protocols
WB protocol for RNASEH2A antibody 67976-1-IgDownload protocol
IHC protocol for RNASEH2A antibody 67976-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols