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  • Featured Product
  • KD/KO Validated

RNASEH2B Polyclonal antibody

RNASEH2B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17527-1-AP

Synonyms

AGS2, DLEU8, Ribonuclease H2 subunit B, ribonuclease H2, subunit B, Ribonuclease HI subunit B, RNase H2 subunit B, RNASEH2B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using RNASEH2B Polyclonal antibody (17527-1-AP)

    WB analysis of mouse thymus using 17527-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using RNASEH2B Polyclonal antibody (17527-1-AP)

    WB analysis of mouse lung using 17527-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RNASEH2B Polyclonal antibody (17527-1-AP)

    WB analysis of HeLa using 17527-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RNASEH2B Polyclonal antibody (17527-1-AP)

    WB analysis of MCF-7 using 17527-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse thymus tissue, HeLa cells, MCF-7 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17527-1-AP targets RNASEH2B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH2B fusion protein Ag11189 相同性解析による交差性が予測される生物種
Full Name ribonuclease H2, subunit B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC036744
Gene symbol RNASEH2B
Gene ID (NCBI) 79621
RRIDAB_10597401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

Protocols

Product Specific Protocols
WB protocol for RNASEH2B antibody 17527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nutr Metab (Lond)

Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.

Authors - Shuo Cong
View Article
humanWB

Cell Rep

Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition

Authors - Yan Li
  • KD Validated
View Article