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RNASEH2C Polyclonal antibody

RNASEH2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16518-1-AP

Synonyms

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C


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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, human spleen tissue, human testis tissue, HeLa cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human lung tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16518-1-AP targets RNASEH2C in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH2C fusion protein Ag9739 相同性解析による交差性が予測される生物種
Full Name ribonuclease H2, subunit C
Calculated molecular weight 164 aa, 18 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC023588
Gene symbol RNASEH2C
Gene ID (NCBI) 84153
RRIDAB_2181648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNASEH2C antibody 16518-1-APDownload protocol
IHC protocol for RNASEH2C antibody 16518-1-APDownload protocol
IP protocol for RNASEH2C antibody 16518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.

Authors - Michal Zimmermann
humanWB

Hum Mol Genet

Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.

Authors - Sara Pizzi
humanWB

J Biol Chem

Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.

Authors - Laura A Lindsey-Boltz
humanWB

Cell Rep

Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition

Authors - Yan Li
  • KD Validated