• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASEL Polyclonal antibody, PBS Only

RNASEL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22577-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RNASEL Polyclonal antibody (22577-1-AP)

    WB analysis using 22577-1-AP (same clone as 22577-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using RNASEL Polyclonal antibody (22577-1-AP)

    WB analysis of RAW 264.7 using 22577-1-AP (same clone as 22577-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using RNASEL Polyclonal antibody (22577-1-AP)

    IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RNASEL Polyclonal antibody (22577-1-AP)

    IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using RNASEL Polyclonal antibody (22577-1-AP)

    IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using RNASEL Polyclonal antibody (22577-1-AP)

    IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNASEL Polyclonal antibody (22577-1-AP)

    IF Staining of HepG2 using 22577-1-AP (same clone as 22577-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNASEL antibody (22577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22577-1-PBS targets RNASEL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEL fusion protein Ag17409 相同性解析による交差性が予測される生物種
Full Name ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Calculated molecular weight 741 aa, 84 kDa
Observed molecular weight 84 kDa; 73 kDa
GenBank accession numberBC115697
Gene symbol RNASEL
Gene ID (NCBI) 6041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.