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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASET2 Recombinant antibody

RNASET2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240581C4

Cat no : 83650-1-RR

Synonyms

RNase T2, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19, bA514O12.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASET2 Recombinant antibody (83650-1-RR)

    WB analysis using 83650-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83650-1-RR (RNASET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83650-1-RR

    Affinity and Kinetic Characterization of 83650-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83650-1-RR against Human RNASET2 were performed. The affinity constant is 2.09 nM.

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Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83650-1-RR is RNASET2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNASET2 fusion protein Ag28353 相同性解析による交差性が予測される生物種
Full Name ribonuclease T2
Calculated molecular weight 256 aa, 29 kDa
Observed molecular weight25-45 kDa
GenBank accession numberBC039713
Gene symbol RNase T2
Gene ID (NCBI) 8635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

Protocols

Product Specific Protocols
WB protocol for RNASET2 antibody 83650-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols