Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, Jurkat cells, human brain tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNF10 fusion protein Ag10433 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 10 |
| Calculated molecular weight | 811 aa, 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC016622 |
| Gene Symbol | RNF10 |
| Gene ID (NCBI) | 9921 |
| RRID | AB_2181846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N5U6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IHC protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IF protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IP protocol for RNF10 antibody 16936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.
| ||
Cell Rep The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.
| ||
Nat Cell Biol Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition | ||
Nat Commun E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance | ||
Mol Neurobiol The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology. |