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  • Featured Product
  • KD/KO Validated

RNF113A Polyclonal antibody

RNF113A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 27018-1-AP

Synonyms

ZNF183, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF113A, Cwc24 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF113A Polyclonal antibody (27018-1-AP)

    WB analysis using 27018-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27018-1-AP (RNF113A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RNF113A Polyclonal antibody (27018-1-AP)

    WB analysis of MCF-7 using 27018-1-AP

    WB result of RNF113A antibody (27018-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF113A transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RNF113A Polyclonal antibody (27018-1-AP)

    IHC staining of human ovary cancer using 27018-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27018-1-AP (RNF113A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHCT 116 cells, MCF-7 cells, HEK-293T cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27018-1-AP targets RNF113A in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF113A fusion protein Ag25313 相同性解析による交差性が予測される生物種
Full Name ring finger protein 113A
Calculated molecular weight 39 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC000832
Gene symbol RNF113A
Gene ID (NCBI) 7737
RRIDAB_2880724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF113A antibody 27018-1-APDownload protocol
IHC protocol for RNF113A antibody 27018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,CoIP

Biomark Res

EIF4A3-induced Circ_0001187 facilitates AML suppression through promoting ubiquitin-proteasomal degradation of METTL3 and decreasing m6A modification level mediated by miR-499a-5p/RNF113A pathway

Authors - Xinyu Yang
View Article
humanWB

Am J Transl Res

Prognostic value of RNF113A shows a correlation with immune infiltrates in colorectal cancer

Authors - Minghui Chen
View Article