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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF123 Polyclonal antibody (25260-1-AP)

    WB analysis using 25260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25260-1-AP (RNF123 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF123 Polyclonal antibody (25260-1-AP)

    IP experiment of HeLa using 25260-1-AP

    IP result of anti-RNF123 (IP:25260-1-AP, 4ug; Detection:25260-1-AP 1:600) with HeLa cells lysate 1200 ug.

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RNF123 Polyclonal antibody

RNF123 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25260-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

ring finger protein 123, KPC1, FP1477

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HuH-7 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25260-1-AP targets RNF123 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF123 fusion protein Ag19653 相同性解析による交差性が予測される生物種
Full Name ring finger protein 123
Calculated molecular weight 1314 aa, 149 kDa
Observed molecular weight149 kDa
GenBank accession numberBC130632
Gene Symbol RNF123
Gene ID (NCBI) 63891
RRIDAB_3669467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5XPI4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RING finger protein 123 (RNF123), also known as KPC1, is an E3 ubiquitin ligase that belongs to the RING-finger family of ligases that contain a 40-60-amino acid long RING domain which is essential for causing substrate ubiquitination (PMID: 29676528). RNF123 promoted TLR3/IRF7-induced type I IFN signaling and inhibited viral replication by degrading SOCS1 (PMID: 37014223).

Protocols

Product Specific Protocols
WB protocol for RNF123 antibody 25260-1-APDownload protocol
IP protocol for RNF123 antibody 25260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols