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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF126 Polyclonal antibody (19835-1-AP)

    WB analysis using 19835-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19835-1-AP (RNF126 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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RNF126 Polyclonal antibody

RNF126 Polyclonal Antibody for WB, ELISA
Cat No. 19835-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ring finger protein 126, RNF126

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19835-1-AP targets RNF126 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF126 fusion protein Ag13816 相同性解析による交差性が予測される生物種
Full Name ring finger protein 126
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC001442
Gene Symbol RNF126
Gene ID (NCBI) 55658
RRIDAB_3085588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

Protocols

Product Specific Protocols
WB protocol for RNF126 antibody 19835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols