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  • Featured Product
  • KD/KO Validated

RNF128 Polyclonal antibody

RNF128 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26015-1-AP

Synonyms

GRAIL, ring finger protein 128, RNF128

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF128 Polyclonal antibody (26015-1-AP)

    WB analysis using 26015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26015-1-AP (RNF128 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RNF128 Polyclonal antibody (26015-1-AP)

    IHC staining of human liver cancer using 26015-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RNF128 Polyclonal antibody (26015-1-AP)

    IHC staining of human liver cancer using 26015-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26015-1-AP is RNF128 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF128 fusion protein Ag22834 相同性解析による交差性が予測される生物種
Full Name ring finger protein 128
Calculated molecular weight 428 aa, 47 kDa
Observed molecular weight 40-47 kDa, 66-70 kDa
GenBank accession numberBC063404
Gene symbol RNF128
Gene ID (NCBI) 79589
RRIDAB_2880336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF128 antibody 26015-1-APDownload protocol
IHC protocol for RNF128 antibody 26015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Res Ther

Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

Authors - Mengqi Chu
  • KD Validated
View Article