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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF133 Polyclonal antibody

RNF133 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20792-1-AP

Synonyms

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNF133 Polyclonal antibody (20792-1-AP)

    WB analysis of HeLa using 20792-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20792-1-AP (RNF133 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of mouse testis using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of mouse testis using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of rat testis using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of rat testis using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20792-1-AP (RNF133 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of human brain using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20792-1-AP (RNF133 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RNF133 Polyclonal antibody (20792-1-AP)

    IHC staining of human brain using 20792-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20792-1-AP (RNF133 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF133 Polyclonal antibody (20792-1-AP)

    IF Staining of HeLa using 20792-1-AP

    Immunofluorescent analysis of Hela cells, using RNF133 antibody 20792-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, human brain tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20792-1-AP targets RNF133 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF133 fusion protein Ag14537 相同性解析による交差性が予測される生物種
Full Name ring finger protein 133
Calculated molecular weight 376 aa, 42 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC022038
Gene symbol RNF133
Gene ID (NCBI) 168433
RRIDAB_10695189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.

Protocols

Product Specific Protocols
WB protocol for RNF133 antibody 20792-1-APDownload protocol
IHC protocol for RNF133 antibody 20792-1-APDownload protocol
IF protocol for RNF133 antibody 20792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols