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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF133 Recombinant antibody

RNF133 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240397C12

Cat no : 83462-1-RR

Synonyms

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF133 Recombinant antibody (83462-1-RR)

    WB analysis using 83462-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83462-1-RR (RNF133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83462-1-RR

    Affinity and Kinetic Characterization of 83462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83462-1-RR against Human RNF133 were performed. The affinity constant is 0.714 nM.

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83462-1-RR is RNF133 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF133 fusion protein Ag14537 相同性解析による交差性が予測される生物種
Full Name ring finger protein 133
Calculated molecular weight 376 aa, 42 kDa
Observed molecular weight47 kDa
GenBank accession numberBC022038
Gene symbol RNF133
Gene ID (NCBI) 168433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.

Protocols

Product Specific Protocols
WB protocol for RNF133 antibody 83462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols