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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using RNF135 Recombinant antibody (84984-1-RR)

    IF Staining of PC-12 using 84984-1-RR (same clone as 84984-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RNF135 antibody (84984-1-RR, Clone: 241606D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84984-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84984-1-RR

    Affinity and Kinetic Characterization of 84984-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84984-1-RR against Human RNF135 were performed. The affinity constant is 49.1 nM.

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RNF135 Recombinant antibody, PBS Only

RNF135 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84984-1-PBS
Clone No.241606D8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, Indirect ELISA

Synonyms

RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF135

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84984-1-PBS targets RNF135 in IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF135 fusion protein Ag18821 相同性解析による交差性が予測される生物種
Full Name ring finger protein 135
Calculated molecular weight 432 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC005084
Gene Symbol RNF135
Gene ID (NCBI) 84282
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IUD6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication.