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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using RNF14 Polyclonal antibody (26368-1-AP)

    WB analysis of HEK-293T using 26368-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RNF14 Polyclonal antibody (26368-1-AP)

    WB analysis of COLO 320 using 26368-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using RNF14 Polyclonal antibody (26368-1-AP)

    IP experiment of COLO 320 using 26368-1-AP

    IP result of anti-RNF14 (IP:26368-1-AP, 4ug; Detection:26368-1-AP 1:600) with COLO 320 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RNF14 Polyclonal antibody (26368-1-AP)

    IHC staining of human colon cancer using 26368-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26368-1-AP (RNF14 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RNF14 Polyclonal antibody (26368-1-AP)

    IF Staining of HEK-293 using 26368-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF14 antibody (26368-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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RNF14 Polyclonal antibody

RNF14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26368-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HRIHFB2038, HFB30, EC:2.3.2.31, E3 ubiquitin-protein ligase RNF14, ARA54

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26368-1-AP targets RNF14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF14 fusion protein Ag23734 相同性解析による交差性が予測される生物種
Full Name ring finger protein 14
Observed molecular weight 47-54 kDa
GenBank accession numberBC126185
Gene Symbol RNF14
Gene ID (NCBI) 9604
RRIDAB_2880494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF14 antibody 26368-1-APDownload protocol
IHC protocol for RNF14 antibody 26368-1-APDownload protocol
IF protocol for RNF14 antibody 26368-1-APDownload protocol
IP protocol for RNF14 antibody 26368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols