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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF141 Polyclonal antibody

RNF141 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26041-1-AP

Synonyms

ring finger protein 141, RNF141, ZFP26, Zinc finger protein 230, ZNF230

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using RNF141 Polyclonal antibody (26041-1-AP)

    WB analysis of mouse testis using 26041-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26041-1-AP (RNF141 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using RNF141 Polyclonal antibody (26041-1-AP)

    WB analysis of mouse testis using 26041-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26041-1-AP (RNF141 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RNF141 Polyclonal antibody (26041-1-AP)

    IP experiment of mouse testis using 26041-1-AP

    IP result of anti-RNF141 (IP:26041-1-AP, 4ug; Detection:26041-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF141 Polyclonal antibody (26041-1-AP)

    IHC staining of human colon using 26041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF141 Polyclonal antibody (26041-1-AP)

    IHC staining of human colon using 26041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF141 Polyclonal antibody (26041-1-AP)

    IHC staining of human colon using 26041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF141 Polyclonal antibody (26041-1-AP)

    IHC staining of human colon using 26041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26041-1-AP (RNF141 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26041-1-AP is RNF141 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF141 fusion protein Ag23316 相同性解析による交差性が予測される生物種
Full Name ring finger protein 141
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC018104
Gene symbol RNF141
Gene ID (NCBI) 50862
RRIDAB_2880348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF141 antibody 26041-1-APDownload protocol
IHC protocol for RNF141 antibody 26041-1-APDownload protocol
IP protocol for RNF141 antibody 26041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols