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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF150 Polyclonal antibody

RNF150 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21438-1-AP

Synonyms

KIAA1214, ring finger protein 150, RNF150

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RNF150 Polyclonal antibody (21438-1-AP)

    WB analysis of mouse lung using 21438-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using RNF150 Polyclonal antibody (21438-1-AP)

    WB analysis of mouse eye using 21438-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RNF150 Polyclonal antibody (21438-1-AP)

    WB analysis of Y79 using 21438-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RNF150 Polyclonal antibody (21438-1-AP)

    WB analysis of mouse brain using 21438-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RNF150 Polyclonal antibody (21438-1-AP)

    WB analysis of human heart using 21438-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF150 Polyclonal antibody (21438-1-AP)

    IHC staining of human colon using 21438-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21438-1-AP (RNF150 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using RNF150 Polyclonal antibody (21438-1-AP)

    IHC staining of human colon using 21438-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21438-1-AP (RNF150 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, human heart tissue, mouse brain tissue, mouse eye tissue, Y79 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21438-1-AP targets RNF150 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF150 fusion protein Ag16064 相同性解析による交差性が予測される生物種
Full Name ring finger protein 150
Calculated molecular weight 438 aa, 48 kDa
Observed molecular weight 43-44 kDa
GenBank accession numberBC101992
Gene symbol RNF150
Gene ID (NCBI) 57484
RRIDAB_10733469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RING finger (RNF) family, containing the N-terminal RING domain, is the largest E3 ubiquitin ligase family with 340 validated human members (PMID: 25609945). RNF150 proteins belong to the Goliath family and are involved in apoptosis and embryonic development (PMID: 19690564). RNF150 might be involved in the progression of GC partly via heparin binding which correlates well with angiogenesis and hematogenous metastasis (PMID: 37528105). There are four isoforms of RNF150, 48kDa, 44kDa, 43kDa, 32 kDa respectively.

Protocols

Product Specific Protocols
WB protocol for RNF150 antibody 21438-1-APDownload protocol
IHC protocol for RNF150 antibody 21438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Cell Biol Int

RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating p38 signaling axis

Authors - Wei Deng
View Article