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Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of rat testis using 24708-1-AP (same clone as 24708-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of human testis using 24708-1-AP (same clone as 24708-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    IHC staining of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    IHC staining of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

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RNF151 Polyclonal antibody, PBS Only

RNF151 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24708-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24708-1-PBS targets RNF151 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF151 fusion protein Ag16606 相同性解析による交差性が予測される生物種
Full Name ring finger protein 151
Calculated molecular weight 245 aa, 27 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC113014
Gene Symbol RNF151
Gene ID (NCBI) 146310
RRIDAB_2879683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2KHN1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.