Validation Data Gallery
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Tested Applications
| Positive WB detected in | BxPC-3 cells, HepG2 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
Product Information
28452-1-AP targets RNF160 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNF160 fusion protein Ag29467 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 160 |
| Observed molecular weight | 180-200 kDa |
| GenBank accession number | BC140790 |
| Gene Symbol | RNF160 |
| Gene ID (NCBI) | 26046 |
| RRID | AB_2881144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94822 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF160 antibody 28452-1-AP | Download protocol |
| IHC protocol for RNF160 antibody 28452-1-AP | Download protocol |
| IF protocol for RNF160 antibody 28452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Rep NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Cell Rep The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins | ||
bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
bioRxiv Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation |