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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF160 Recombinant antibody (82926-1-RR)

    WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RNF160 Recombinant antibody (82926-1-RR)

    IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using RNF160 Recombinant antibody (82926-1-RR)

    FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82926-1-RR

    Affinity and Kinetic Characterization of 82926-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

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RNF160 Recombinant antibody, PBS Only

RNF160 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82926-1-PBS
Clone No.230075H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82926-1-PBS targets RNF160 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF160 fusion protein Ag29467 相同性解析による交差性が予測される生物種
Full Name ring finger protein 160
Observed molecular weight180-200 kDa
GenBank accession numberBC140790
Gene Symbol RNF160
Gene ID (NCBI) 26046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94822
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).